Prevention of ischemia-induced hearing loss by intravenous administration of hydrogen-rich saline in gerbil

Hideo Ogawa, Kiyofumi Gyo, Masachika Shudou, Masahiro Okada, Naohito Hato

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DOI: 10.1016/j.neulet.2017.12.013 DOI is the universal ID for this study.

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Objective: Hydrogen-rich water, which is a potent antioxidant agent, was investigated for its protective effects against ischemic damage of the cochlea in gerbils.

Methods: The animals were subjected to transient cochlear ischemia by occluding the bilateral vertebral arteries for l5min. Five milliliters of hydrogen-rich saline was then intravenously administered immediately after the insult. Saline without hydrogen was used as a control. Effects of hydrogen were evaluated using the auditory brainstem response (ABR) and histological studies of the inner ear.

Results: In non-ischemia animals, ABR thresholds and histological findings of the cochlea did not change by administration of saline or hydrogen-rich saline. In contrast, transient cochlear ischemia caused a 24.2±3.8dB increase in the ABR threshold at 8kHz, and a decrease of 14.1%±1.8% in the number of inner hair cells (IHCs) at the basal turn on day 7. Ischemic damage was more severe at 16 and 32kHz. When the animals were treated with hydrogen-rich saline, cochlear damage was significantly reduced: the increase in ABR threshold was 11.7±2.6dB at 8kHz and the IHC loss was 7.5%±2.1% at the basal turn on day 7. The effects of hydrogen-rich saline were more prominent at higher frequencies. Conclusions: Intravenous administration of hydrogen-rich saline was effective in preventing acute hearing loss due to transient cochlear ischemia.

Publish Year 2017
Country Japan
Rank Positive
Journal Neuroscience Letters
Primary Topic Ear
Secondary TopicHearing Loss
Model Gerbil
Tertiary TopicIschemia-Reperfusion Injury
Vehicle Saline (Dissolved)
pH Neutral
Application Injection