Molecular hydrogen suppresses Porphyromonas gingivalis lipopolysaccharide-induced increases in interleukin-1 alpha and interleukin-6 secretion in human gingival cells
Akira Matsumoto, Daigo Matsuoka, Nene Yonekura, Yasukazu Saitoh
Abstract:
Periodontitis is defined as a multifactorial polymicrobial infection accompanied by inflammatory reactions. Porphyromonas gingivalis (Pg) is known as a major pathogen in the initiation and progression of periodontitis, and a major virulence factor is Pg lipopolysaccharide (LPS). Molecular hydrogen (H2) has been reported to act as a gaseous antioxidant, which suppresses periodontitis progression by decreasing gingival oxidative stress. However, no human periodontitis model has examined the anti-inflammatory effects of H2. In this study, we examined the effects of H2 on Pg LPS-induced secretion of 8 types of inflammation markers in a human periodontitis model using human gingival cells with enzyme-linked immunosorbent assays. Our results demonstrated that Pg LPS increased interleukin (IL) 1 alpha (IL-1α) and IL-6 secretion, but H2 significantly suppressed the secretion of both cytokines without cytotoxicity. H2 can suppress the production of IL-1α and IL-6, which are identified as cytokines involved in inflammatory reactions in periodontal disease. Thus, H2 may provide therapeutic applications for periodontitis.
| Publish Year |
2021 |
| Country |
Japan |
| Rank |
Positive |
| Journal |
Molecular and Cellular Biochemistry |
| Primary Topic |
Mouth |
| Secondary Topic | Periodontitis |
| Model |
Cell Culture |
| Tertiary Topic | Inflammation |
| Vehicle |
Water (Electrolysis) |
| pH |
Neutral |
| Application |
Culture Media |
| Comparison |
|
| Complement |
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