Molecular Hydrogen Alleviates Cellular Senescence in Endothelial Cells

Fumihiko Hara, Ippei Watanabe, Junichi Yamazaki, Junko Tatebe, Takanori Ikeda, Toshisuke Morita

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DOI: 10.1253/circj.CJ-16-0227 DOI is the universal ID for this study.

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Abstract:

Background: Substantial evidence indicates that molecular hydrogen (H2) has beneficial vascular effects because of its antioxidant and/or anti-inflammatory effects. Thus, hydrogen-rich water may prove to be an effective anti-aging drink. This study examined the effects of H2on endothelial senescence and clarified the mechanisms involved. Methods and results: Hydrogen-rich medium was produced by a high-purity hydrogen gas generator. Human umbilical vein endothelial cells (HUVECs) were incubated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) for various time periods in normal or hydrogen-rich medium. The baseline H2concentration in hydrogen-rich medium was 0.55±0.07 mmol/L. This concentration gradually decreased, and H2was almost undetectable in medium after 12 h. At 24 h after TCDD exposure, HUVECs treated with TCDD exhibited increased 8OHdG and acetyl-p53 expression, decreased nicotinamide adenine dinucleotide (NAD(+))/NADH ratio, impaired Sirt1 activity, and enhanced senescence-associated β-galactosidase. However, HUVECs incubated in hydrogen-rich medium did not exhibit these TCDD-induced changes accompanying Nrf2 activation, which was observed even after H2was undetectable in the medium. Chrysin, an inhibitor of Nrf2, abolished the protective effects of H2on HUVECs. Conclusions: H2has long-lasting antioxidant and anti-aging effects on vascular endothelial cells through the Nrf2 pathway, even after transient exposure to H2. Hydrogen-rich water may thus be a functional drink that increases longevity. (Circ J 2016; 80: 2037-2046).

Publish Year 2016
Country Japan
Rank Positive
Journal Circulation Journal
Primary Topic Heart
Secondary TopicAging
Model Cell Culture
Tertiary TopicOxidative Stress
Vehicle Medium (Dissolved)
pH Neutral
Application Culture Media
Comparison
Complement