Maternal molecular hydrogen administration on lipopolysaccharide-induced mouse fetal brain injury
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DOI:
10.3164/jcbn.15-90
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Abstract:
Fetal brain injury is often related to prenatal inflammation; however, there is a lack of effective therapy. Recently, molecular hydrogen (H2), a specific antioxidant to hydroxyl radical and peroxynitrite, has been reported to have anti-inflammatory properties. The aim of this study was to investigate whether maternal H2 administration could protect the fetal brain against inflammation. Pregnant C3H/HeN mice received an intraperitoneal injection of lipopolysaccharide (LPS) on gestational day 15.5 and were provided with H2 water for 24 h prior to LPS injection. Pup brain samples were collected on gestational day 16.5, and the levels of apoptosis and oxidative damage were evaluated using immunohistochemistry. Interleukin-6 (IL-6) levels were examined using real-time PCR. The levels of apoptosis and oxidative damage, as well as the levels of IL-6 mRNA, increased significantly when the mother was injected with LPS than that in the control group. However, these levels were significantly reduced when H2 was administered prior to the LPS-injection. Our results suggest that LPS-induced apoptosis, oxidative damage and inflammation in the fetal brain were ameliorated by maternal H2 administration. Antenatal H2 administration might protect the premature brain against maternal inflammation.| Publish Year | 2015 |
|---|---|
| Country | Japan |
| Rank | Positive |
| Journal | Journal of Clinical Biochemistry and Nutrition |
| Primary Topic | Pregnancy |
| Secondary Topic | Neuroprotection |
| Model | Mouse |
| Tertiary Topic | Uterine Inflammation |
| Vehicle | Water, Commercial (Blue Mercury) |
| pH | Neutral |
| Application | Ingestion |
| Comparison | |
| Complement |