Hydrogen Treatment Protects Mice Against Chronic Pancreatitis by Restoring Regulatory T Cells Loss

Chao Ma, Jianping Lu, Jing Li, Li Su, Luguang Chen, Tiegong Wang, Yun Bian

Read more:

DOI: 10.1159/000485906 DOI is the universal ID for this study.

This link will take you to the full study.

Abstract:

Background/aims: Chronic pancreatitis is an inflammatory disease of the pancreas characterized by progressive tissue destruction and fibrogenesis. The development of chronic pancreatitis is associated with immune cell dysregulation. Currently, the specific and effective treatment of chronic pancreatitis remains absent.

Methods: By using an L-arginine induced chronic pancreatitis mouse model, we tested the therapeutic potential of hydrogen, a strong hydroxyl radicals scavenger, in the chronic pancreatitis model. Tissue inflammation, damage and fibrosis were analyzed on HE, TUNEL, MPO, and sirius staining. Pancreas levels of MDA content, SOD activity, TNF-α , IL-10 cytokine expression and serum amylase and lipase activity were determined by ELISA and absorbance assay. Apoptosis, T cells subtype proportion and intracellular level of reactive oxygen species (ROS) were analyzed by flow cytometry. Tregs adoptive transfer and CD25 neutralization were used to validate the role of Tregs in chronic pancreatitis.

Results: We found that hydrogen treatment significantly improved multiple symptoms of chronic pancreatitis. The number of Tregs was reduced in chronic pancreatitis mice, while hydrogen treatment restored the Treg loss by L-arginine administrations. Depletion of Tregs abolished the protective effect of hydrogen treatment in chronic pancreatitis. In vitro study showed that hydrogen blocked ROS generation in Tregs and promoted Tregs survival.

Conclusion: Hydrogen treatment showed reliable benefits in controlling the severity of chronic pancreatitis. Our study supported that hydrogen could be used as a novel treatment in chronic pancreatitis patient in the future.


Publish Year 2017
Country China
Rank Positive
Journal Cellular Physiology and Biochemistry
Primary Topic Pancreas
Secondary TopicPancreatitis
Model Mouse
Tertiary TopicImmune Dysfunction
Vehicle Gas
pH N/A
Application Inhalation
Comparison
Complement

Related Articles

Effectiveness of Acidic Oxidative Potential Water in Preventing Bacterial Infection in Islet ...

1999 - Pancreas - Surgery/Transplantation

At a number of points in the current procedures of islet isolation and islet culture after the harvesting of donor pancreata, microorganisms could potentially infect the islet preparation. Furthermore, the use of islets from multiple donors can ...

Protective mechanism of reduced water against alloxan-induced pancreatic beta-cell damage: ...

2002 - Pancreas - Diabetes (Type I)

Reactive oxygen species (ROS) cause irreversible damage to biological macromolecules, resulting in many diseases. Reduced water (RW) such as hydrogen-rich electrolyzed reduced water and natural reduced waters like Hita Tenryosui water in Japan and ...

Effect of Reduced Water on the Apoptotic Cell Death Triggered by Oxidative Stress in Pancreatic β ...

2005 - Pancreas - Diabetes (Type I)

Recent studies have demonstrated that reactive oxygen species9ROS9and the resulting oxidative stress play an important role in apoptosis. Apoptosis is implicated in pathophysiology of diabetes mellitus. Antioxidants can block or delay apoptosis. We ...

Hydrogen-rich saline ameliorates the severity of l-arginine-induced acute pancreatitis in rats

2010 - Pancreas - Pancreatitis

Molecular hydrogen, which reacts with the hydroxyl radical, has been considered as a novel antioxidant. Here, we evaluated the protective effects of hydrogen-rich saline on the l-arginine (l-Arg)-induced acute pancreatitis (AP). AP was induced in ...

Hydrogen-rich saline reduces the oxidative stress and relieves the severity of trauma-induced acute ...

2012 - Pancreas - Pancreatitis

Currently, little evidence exists to support whether the therapeutic approaches for treating ordinary acute pancreatitis (AP) are effective in trauma-induced pancreatitis. Hydrogen-rich (H2) saline is an antioxidant treatment capable of ameliorating ...

Effects of Hydrogen-Rich Saline on Taurocholate-Induced Acute Pancreatitis in Rat

2013 - Pancreas - Pancreatitis

Oxidative stress plays an important role in the pathogenesis of acute pancreatitis (AP). As an ideal exterminator of poisonous free radicals, hydrogen can clearly reduce the degree of oxidative damage caused by severe acute pancreatitis (SAP) and ...