Hydrogen-rich and hyperoxygenate saline inhibits lipopolysaccharide-induced lung injury through mediating NF-κB/NLRP3 signaling pathway in C57BL/6 mice

Hao Xu, Jian Wang, Jiankang Liu, Ke Cao, Yingying Fan, Zhihui Feng

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DOI: 10.1002/tox.23507 DOI is the universal ID for this study.

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Background: Acute lung injury (ALI) is one kind of frequently occurred emergency in Intensive Care Unite with a high mortality. The underlying causes are uncontrolled inflammatory reactions and intractable hypoxemia, which are difficult to control and improve. In the past 10 years, gas medical studies have found that both hydrogen molecules and oxygen molecules have protective effects on acute lung injury by improving inflammatory reactions and hypoxia, respectively. Oxygen is an oxidant and hydrogen is an antioxidant. In this study, we investigated the combined effect of above two-gas molecular on lipopolysaccharide (LPS) -induced acute lung injury.

Methods: To clarify whether the combination of hydrogen and oxygen could increase or cancel out the protective effect, an ALI mice model induced by intraperitoneal injection of LPS was established, and the degree of lung tissue and mitochondria damage was evaluated based on the pathological sections, inflammatory factors, wet-dry ratio, bronchoalveolar lavage fluid (BALF). Immunohistochemistry, electron microscopy, western blotting and other detection methods also used to evaluate the therapeutic effect on acute lung injury model.

Results: We observed that the combined protective effect of hydrogen and oxygen was superior to their respective protective effects, and the specific molecular mechanisms of the two therapies might be different.

Conclusion: Hydrogen plays a more important role in the inflammatory and anti-apoptosis mechanisms, while oxygen improves hypoxia of the body, and thus, its molecular mechanism may be closely associated to the hypoxia pathways.

Publish Year 2022
Country China
Rank Positive
Journal Environmental Toxicology
Primary Topic Lung
Secondary TopicSepsis
Model Mouse
Tertiary TopicLung Injury
Vehicle Gas
pH N/A
Application Inhalation
Comparison Oxygen
Complement Oxygen