Hydrogen from intestinal bacteria is protective for Concanavalin A-induced hepatitis

K.T. Shanmugam, Kazuhisa Ouhara, Kimihiro Sato, Marcelo J. B. Silva, Mikihito Kajiya, Phi M. Do, Toshihisa Kawai

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DOI: 10.1016/j.bbrc.2009.06.024 DOI is the universal ID for this study.

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It is well known that some intestinal bacteria, such as Escherichia coli, can produce a remarkable amount of molecular hydrogen (H(2)). Although the antioxidant effects of H(2) are well documented, the present study examined whether H(2) released from intestinally colonized bacteria could affect Concanavalin A (ConA)-induced mouse hepatitis. Systemic antibiotics significantly decreased the level of H(2) in both liver and intestines along with suppression of intestinal bacteria. As determined by the levels of AST, ALT, TNF-alpha and IFN-gamma in serum, suppression of intestinal bacterial flora by antibiotics increased the severity of ConA-induced hepatitis, while reconstitution of intestinal flora with H(2)-producing E. coli, but not H(2)-deficient mutant E. coli, down-regulated the ConA-induced liver inflammation. Furthermore, in vitro production of both TNF-alpha and IFN-gamma by ConA-stimulated spleen lymphocytes was significantly inhibited by the introduction of H(2). These results indicate that H(2) released from intestinal bacteria can suppress inflammation induced in liver by ConA.

Publish Year 2009
Country United States
Rank Positive
Journal Biochemical and Biophysical Research Communications
Primary Topic Liver
Secondary TopicAutoimmune
Model Mouse
Tertiary TopicGut Microbiome
Vehicle Water (Dissolved)
pH Neutral
Application Ingestion