Conflicting findings on the effectiveness of hydrogen therapy for ameliorating vascular leakage in a 5-day post hypoxic-ischemic survival piglet model

Eri Inoue, Kenichi Ohta, Kosuke Koyano, Kota Inoue, Makoto Arioka, Masaki Ueno, Shinji Nakamura, Takanori Miki, Takashi Kusaka, Takayuki Yokota, Toi Tsuchiya, Tsutomu Mitsuie, Yasuhiro Nakao, Yinmon Htun, Yukihiko Konishi

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DOI: 10.1038/s41598-023-37577-0 DOI is the universal ID for this study.

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Neonatal hypoxic-ischemic encephalopathy (HIE) is a major cause of morbidity and mortality in newborns in both high- and low-income countries. The important determinants of its pathophysiology are neural cells and vascular components. In neonatal HIE, increased vascular permeability due to damage to the blood-brain barrier is associated with seizures and poor outcomes in both translational and clinical studies. In our previous studies, hydrogen gas (H2) improved the neurological outcome of HIE and ameliorated the cell death. In this study, we used albumin immunohistochemistry to assess if H2 inhalation effectively reduced the cerebral vascular leakage. Of 33 piglets subjected to a hypoxic-ischemic insult, 26 piglets were ultimately analyzed. After the insult, the piglets were grouped into normothermia (NT), H2 ventilation (H2), therapeutic hypothermia (TH), and H2 combined with TH (H2-TH) groups. The ratio of albumin stained to unstained areas was analyzed and found to be lower in the H2 group than in the other groups, although the difference was not statistically significant. In this study, H2 therapy did not significantly improve albumin leakage despite the histological images suggesting signs of improvement. Further investigations are warranted to study the efficacy of H2 gas for vascular leakage in neonatal HIE.

Publish Year 2023
Country Japan
Rank Neutral
Journal Nature Scientific Reports
Primary Topic Heart
Secondary TopicPregnancy
Model Pig
Tertiary TopicHypoxia-Ischemia
Vehicle Gas
pH N/A
Application Inhalation