Molecular hydrogen decelerates rheumatoid arthritis progression through inhibition of oxidative stress
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PMID: 27830032
Abstract:
Rheumatoid arthritis (RA) is a chronic inflammatory disease which results in progressive destruction of the joint. In this study, we examined if the hydrogen could inhibit inflammation in a mouse model of collagen-induced arthritis (CIA) via oxidative stress on RA-FLSs. Moreover, to identify the mechanisms of action, we evaluated the effect of hydrogen on RA-FLSs development and the expression of pro-inflammatory cytokines and signaling pathways. Based on our result, H2 enriched medium can increase super oxide dismutase (SOD) level following H2O2 treatment and decrease 8-hydroxy-2'-deoxyguanosine (8-OHdG) level. Since H2O2 treatment activates MAPK, NF-κB and TGF-β1 in cells, our study suggested that H2 could inhibit H2O2 activated MAPK and NF-κB activation as well as TGF-β1 expression in treated cells. Taken together, our data suggested that H2 can directly neutralize OH and ONOO- to reduce oxidative stress. Moreover, MAPK and NF-κB pathway also play roles in oxidative damage caused by H2O2 in RA-FLSs. H2 can provide protection to cells against inflammation, which may be related to inhibition of the activation of MAPK and NF-κB.| Publish Year | 2016 |
|---|---|
| Country | China |
| Rank | Positive |
| Journal | American Journal of Translational Research |
| Primary Topic | Bone |
| Secondary Topic | Rheumatoid Arthritis |
| Model | Mouse |
| Tertiary Topic | Oxidative Stress |
| Vehicle | Water (Dissolved) |
| pH | Neutral |
| Application | Injection |
| Comparison | |
| Complement |