Hydrogen-Rich Saline Ameliorates Hepatic Ischemia-Reperfusion Injury Through Regulation of Endoplasmic Reticulum Stress and Apoptosis
Read more:
DOI:
10.1007/s10620-017-4811-8
DOI is the universal ID for this study.
This link will take you to the full study.
Abstract:
Objective: To evaluate the effect of hydrogen-rich saline (HS) on hepatic ischemia-reperfusion (I/R) injury.Methods: Forty rats were randomly allocated into five groups: one sham group (control group), one group treated with 20 min of ischemia and normal saline (NS; I/R1 + NS group), one group treated with 20 min of ischemia and HS (I/R1 + HS group), one group treated with 60 min of ischemia and NS (I/R2 + NS group), and one group treated with 60 min of ischemia and HS (I/R2 + HS group). After reperfusion for 6 h, hepatic function, oxidative stress, pathological changes, and apoptosis of hepatic cells were evaluated. Furthermore, the expression levels of endoplasmic reticulum (ER) stress-associated proteins were identified.
Results: Serum ALT and AST levels and tissue MDA content in the I/R + HS groups were significantly lower than those in the I/R + NS groups. Pathological changes were also significantly ameliorated in the HS groups compared with those in the NS groups. Moreover, HS appeared to significantly attenuate hepatic I/R-induced ER stress responses, as indicated by the decreased expression of C/EBP homologous protein, protein-kinase-RNA-like ER kinase, and inositol-requiring protein-1α, as well as the increased expression of GRP78 proteins. Finally, the levels of apoptotic markers such as caspase-3 and TUNEL-positive cells were significantly lower in the HS groups than in the NS control groups, whereas the level of Bcl2 protein increased in the HS groups.
Conclusion: The protective effect of HS can be attributed to ER stress and apoptosis inhibition.
| Publish Year | 2017 |
|---|---|
| Country | China |
| Rank | Positive |
| Journal | Digestive Diseases and Sciences |
| Primary Topic | Liver |
| Secondary Topic | Surgery/Transplantation |
| Model | Rat |
| Tertiary Topic | Ischemia-Reperfusion Injury |
| Vehicle | Saline (Dissolved) |
| pH | Neutral |
| Application | Injection |
| Comparison | |
| Complement |