Hydrogen water alleviates paraquat-induced lung fibroblast injury in vitro by enhancing Nrf2 expression
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DOI:
10.12122/j.issn.1673-4254.2020.02.15
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Abstract:
Objective: To investigate the effects of hydrogen water on proliferation, differentiation, collagen secretion and Nrf2 expression in paraquat-induced human lung fibroblasts.Methods: In vitro cultured human lung fibroblasts (HFL1) exposed to 600 μmol/L paraquat (PQ) for 24 h were treated with hydrogen water with or without RNA interference of Nrf2 expression. The changes in the cell proliferation were examined using MTT assay, and the expressions of Col-I, Col-III, α-SMA and Nrf2 in the cells were detected using Western blotting, real-time quantitative PCR and immunofluorescence assay. The contents of SOD, CAT and GSH in the cells were determined with ELISA.
Results: Compared with the PQ-exposed cells, the cells with hydrogen water treatment showed significantly lowered expressions of Col-I, Col-III, and α-SMA. Interference of Nrf2 expression obviously attenuated the effect of hydrogen water on PQ-exposed cells. Hydrogen water treatment significantly increased the expression of Nrf2 and promoted the production of the antioxidants in PQ-exposed lung fibroblasts. Conclusions: Hydrogen water enhances Nrf2 expression to promote the proliferation and production of antioxidants and inhibit the differentiation and collagen secretion in PQ-exposed human lung fibroblasts in vitro.
| Publish Year | 2020 |
|---|---|
| Country | China |
| Rank | Positive |
| Journal | Journal of Southern Medical University |
| Primary Topic | Lung |
| Secondary Topic | Environmental Pollution |
| Model | Cell Culture |
| Tertiary Topic | Pesticide Toxicity (Paraquat) |
| Vehicle | Water (Dissolved) |
| pH | Neutral |
| Application | Culture Media |
| Comparison | |
| Complement |